Activity

Currently finished projects:

1. Unique biosynthetic enzymes as the key to novel bioactive compounds

Effective search for producers of novel, pharmaceutically attractive natural compounds requires application of new screening techniques using genomic data. Growing information on the genetics of biosynthesis of natural compounds and their structural components, brings the possibility to use newly characterized enzymatic systems as markers in genetic screening of potential producers of structurally and functionally unique active metabolites, which may find it application as novel antibiotics, immunomodulators and cancerostatics.  Two model enzymes of actinomycetes, the cyclizing aminolevulinate synthase and a novel type II polyketides synthase specific for short linear polyketide chains, will be employed in the project.

Aims of the project: 1) By means of genetic screening targeting two novel biosynthetic genes to search for potential producers of novel active compounds. 2) To analyse the biosynthetic gene clusters, which contain the target genes and identify the encoded compounds. 3) To assay biological activities of new metabolites.

Funding: Ministry of Healthcare of the Czech Republic (2017-2020), No. 17-30091A

Principal investigator: Mgr. Kateřina Petříčková, PhD., University of South Bohemia in České Budějovice, Faculty of Science, České Budějovice

Co-investigators: 

RNDr. Alica Chroňáková, PhD., Biology Centre CAS, Institute of Soil Biology, České Budějovice

Ing. Miroslav Petříček, PhD. The First Faculty of Medicine, Charles University in Prague

Outputs:

Lara A. C., Corretto E., Kotrbová L., Lorenc F., Petříčková K., Grabic R., Chroňáková A. (2021)  The Genome Analysis of the Human Lung-Associated Streptomyces sp. TR1341 Revealed the Presence of Beneficial Genes for Opportunistic Colonization of Human Tissues. Microorganisms, 9(8), 1547.

DOI: 10.3390/microorganisms9081547

Gorniaková D., Petříček M., Kahoun D., Grabic R., Zelenka T., Chroňáková A., Petříčková K. (2021) Activation of a cryptic manumycin-type biosynthetic gene cluster of Saccharothrix espanaensis DSM44229 by series of genetic manipulations. Microorganisms 9: Article number 559.
DOI: 10.3390/microorganisms9030559

Herbrík A., Corretto E.Chroňáková A., Langhansová H., Petrásková P., Hrdý J., Čihák M., Krištůfek V., Bobek J., Petříček M., Petříčková K. (2020) A human lung-associated Streptomyces sp. TR1341 produces various secondary metabolites responsible for virulence, cytotoxicity and modulation of immune response. Frontiers in Microbiology 10: Article number 3028.
DOI: 10.3389/fmicb.2019.03028

Hrdý J., Súkeníková L., Petrásková P., Novotná O., Kahoun D., Petříček M., Chroňáková A., Petříčková K. (2020) Inhibition of pro-inflammatory cytokines by metabolites of streptomycetes—a potential alternative to current anti-in?ammatory drugs? Microorganisms 8: Article number 621.
DOI: 10.3390/microorganisms8050621

Project team (ISB):

RNDr. Alica Chroňáková, PhD. 

Dr. Erika Corretto, PhD. (alumni)

Dr. Ana Catalina Lara Rodriguez, PhD.

Ing. Václav Krištůfek, CSc.

Mgr. František Lorenc, PhD. (alumni)

Ing. Martina Petrlíková,

Ing. Lucie Kotrbová

Students:

Linda Jernej (bachelor program Biological Chemistry, Faculty of Science South Bohemian University in České Budějovice)

Theresa Wurzer (bachelor program Biological Chemistry, Faculty of Science South Bohemian University in České Budějovice)

Lisa Pulferer (bachelor program Biological Chemistry, Faculty of Science South Bohemian University in České Budějovice)

Moritz Keller (bachelor program Biological Chemistry, Faculty of Science South Bohemian University in České Budějovice)

2. Towards novel bioactive compounds: characterization of specialized metabolites encoded on unique biosynthetic gene clusters

The development of strategies to activate orphan biosynthetic machineries is one of the major challenges in current natural product research. Polyketide synthases (PKS) are multi-enzyme proteins responsible for the assembly of diverse secondary metabolites of high economic and therapeutic importance. Relevant methods include direct cloning using transformation-assisted recombination in yeast, heterologous pathway expression, mass spectral molecular networking and Red/ET-recombineering to manipulate cluster–specific regulation machineries.

Funding:  Czech Academy of Sciences - DAAD project (2018-2019), No. DAAD-18-02

Principal investigators:

RNDr. Alica Chroňáková, PhD., Biology Centre CAS, Institute of Soil Biology, České Budějovice

prof. Leonard Kaysser, Ph.D. Eberhard-Karls-Universität Tübingen, Inst. Pharmaceutical Biology, Germany

Project team (ISB):

RNDr. Alica Chroňáková, PhD.

Dr. Erika Corretto, PhD. (alumni)

Ing. Václav Krištůfek, CSc.

Mgr. František Lorenc, PhD. (alumni)