Our Team

<

Head: Ing. Václav Krištůfek, CSc. (kristuf@upb.cas.cz)

Curator: RNDr. Alica Chroňáková, PhD. (alicach@upb.cas.cz)

Researchers:
RNDr. Alica Chroňáková, PhD.,
Ing. Václav Krištůfek, CSc.,
RNDr. Tomáš Chrudimský, PhD.

Research assistants: RNDr. Anna Koubová, PhD.

Technicians: ing. Martina Petrlíková

 

Currently running project:

Unique biosynthetic enzymes as the key to novel bioactive compounds

Effective search for producers of novel, pharmaceutically attractive natural compounds requires application of new screening techniques using genomic data. Growing information on the genetics of biosynthesis of natural compounds and their structural components, brings the possibility to use newly characterized enzymatic systems as markers in genetic screening of potential producers of structurally and functionally unique active metabolites, which may find it application as novel antibiotics, immunomodulators and cancerostatics.  Two model enzymes of actinomycetes, the cyclizing aminolevulinate synthase and a novel type II polyketides synthase specific for short linear polyketide chains, will be employed in the project.

Aims of the project: 1) By means of genetic screening targeting two novel biosynthetic genes to search for potential producers of novel active compounds. 2) To analyse the biosynthetic gene clusters, which contain the target genes and identify the encoded compounds. 3) To assay biological activities of new metabolites.

Funding: Ministry of Healthcare of the Czech Republic (2017-2020), No. 17-30091A

Principal investigator: Mgr. Kateřina Petříčková, PhD., University of South Bohemia in České Budějovice, Faculty of Science, České Budějovice

Co-investigators: RNDr. Alica Chroňáková, PhD., Biology Centre CAS, Institute of Soil Biology, České Budějovice

Project team (ISB):

RNDr. Alica Chroňáková, PhD., 

RNDr. Tomáš Chrudimský, PhD.,

Ing. Václav Krištůfek, CSc.,

Ing. Martina Petrlíková

We are seeking for motivated students to attend our team!